Near-optimal conditions for the in vitro culture of hemopoietic progenitor cels in bone marrow from the rat.

Molyneux, G., Rizzo, S., Turton, J.A., Phul, P. and Gibson, F.M. (2009) Near-optimal conditions for the in vitro culture of hemopoietic progenitor cels in bone marrow from the rat. Toxicologic Pathology, 37 (2). pp. 170-174. 10.1177/0192623308328133.

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DOI: 10.1177/0192623308328133

Abstract

In vitro techniques for the culture of hemopoietic stem cells and committed hemopoietic progenitor cells in rat bone marrow have not been adequately described in the literature. In the present investigations, and using commercially available hemopoietic cytokines and growth factors, the conditions required to perform long-term bone marrow culture (LTBMC) using rat femoral bone marrow were studied, in conjunction with the colony-forming unit cell assay (CFU-C), to quantify the number of progenitor cells. CFU-C production by LTBMCs, set up using Iscove’s modified Dulbecco’s medium supplemented with fetal calf serum and horse serum, ceased after week 2 of culture. However, the duration of CFU-C production was significantly increased by supplementing LTBMCs with the cytokine recombinant mouse stem cell factor or recombinant rat stem cell factor.

Item Type:Article
Uncontrolled Keywords:long-term bone marrow culture rat hemopoietic progenitor cells colony-forming unit cell assay mouse stem cell factor in vitro culture
Departments, units and centres:Department of Pharmacology > Centre for Toxicology
ID Code:1222
Journal or Publication Title:Toxicologic Pathology
Deposited By:Library Staff
Deposited On:23 Jun 2009 13:33
Last Modified:24 Feb 2012 11:16

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