Molyneux, G., Rizzo, S., Turton, J.A., Phul, P. and Gibson, F.M. (2009) Near-optimal conditions for the in vitro culture of hemopoietic progenitor cels in bone marrow from the rat. Toxicologic Pathology, 37 (2). pp. 170-174. 10.1177/0192623308328133.
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In vitro techniques for the culture of hemopoietic stem cells and committed hemopoietic progenitor cells in rat bone marrow have not been adequately described in the literature. In the present investigations, and using commercially available hemopoietic cytokines and growth factors, the conditions required to perform long-term bone marrow culture (LTBMC) using rat femoral bone marrow were studied, in conjunction with the colony-forming unit cell assay (CFU-C), to quantify the number of progenitor cells. CFU-C production by LTBMCs, set up using Iscove’s modified Dulbecco’s medium supplemented with fetal calf serum and horse serum, ceased after week 2 of culture. However, the duration of CFU-C production was significantly increased by supplementing LTBMCs with the cytokine recombinant mouse stem cell factor or recombinant rat stem cell factor.
|Uncontrolled Keywords:||long-term bone marrow culture rat hemopoietic progenitor cells colony-forming unit cell assay mouse stem cell factor in vitro culture|
|Departments, units and centres:||Department of Pharmacology > Centre for Toxicology|
|Journal or Publication Title:||Toxicologic Pathology|
|Deposited By:||Library Staff|
|Deposited On:||23 Jun 2009 13:33|
|Last Modified:||24 Feb 2012 11:16|
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