Melzer, N., Villman, C., Becker, K., Harvey, K., Harvey, R.J., Vogel, N., Kluck, C.J., Kneussel, M. and Becker, C.M. (2010) Multifunctional basic motif in the glycine receptor intracellular domain induces subunit-specific sorting. Journal of Biological Chemistry, 285 (6). pp. 3730-3739. 10.1074/jbc.M109.030460 .
Full text not available from this repository.
Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823514
The strychnine-sensitive glycine receptor (GlyR) is a ligand-gated ion channel that mediates fast synaptic inhibition in the vertebrate central nervous system. As a member of the family of Cys-loop receptors, it assembles from five homologous subunits (GlyRalpha1-4 and -beta). Each subunit contains an extracellular ligand binding domain, four transmembrane domains (TM), and an intracellular domain, formed by the loop connecting TM3 and TM4 (TM3-4 loop). The TM3-4 loops of the subunits GlyRalpha1 and -alpha3 harbor a conserved basic motif, which is part of a potential nuclear localization signal. When tested for functionality by live cell imaging of green fluorescent protein and beta-galactosidase-tagged domain constructs, the TM3-4 loops of GlyRalpha1 and -alpha3, but not of GlyRalpha2 and -beta, exhibited nuclear sorting activity. Subunit specificity may be attributed to slight amino acid alterations in the basic motif. In yeast two-hybrid screening and GST pulldown assays, karyopherin alpha3 and alpha4 were found to interact with the TM3-4 loop, providing a molecular mechanism for the observed intracellular trafficking. These results indicate that the multifunctional basic motif of the TM3-4 loop is capable of mediating a karyopherin-dependent intracellular sorting of full-length GlyRs.
|Additional Information:||Full text available electronically from the School of Pharmacy Library.|
|Departments, units and centres:||Department of Pharmacology > Department of Pharmacology|
|Journal or Publication Title:||Journal of Biological Chemistry|
|Deposited By:||Library Staff|
|Deposited On:||04 Aug 2010 10:21|
|Last Modified:||06 Oct 2011 16:01|
Item downloaded times since 04 Aug 2010 10:21.
Repository Staff Only: Item control page
School of Pharmacy Staff Only: Edit a copy to replace this item