A G-rich sequence with the c-kit oncogene promoter forms a parallel G-quadruplex having asymmetric G-tetrad dynamics.

Hsu, S.T.D., Varnai, P., Reszka, A.P., Neidle, S. and Balasubramanian, S. (2009) A G-rich sequence with the c-kit oncogene promoter forms a parallel G-quadruplex having asymmetric G-tetrad dynamics. Journal of the American Chemical Society, 131 (37). pp. 13399-13409. 10.1021/ja904007p.

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DOI: 10.1021/ja904007p

Abstract

Guanine-rich DNA sequences with the ability to form quadruplex structures are enriched in the promoter regions of protein-coding genes, particularly those of proto-oncogenes. G-quadruplexes are structurally polymorphic and their folding topologies can depend on the sample conditions. We report here on a structural study using solution state NMR spectroscopy of a second G-quadruplex-forming motif (c-kit2) that has been recently identified in the promoter region of the c-kit oncogene. In the presence of potassium ions, c-kit2 exists as an ensemble of structures that share the same parallel-stranded propeller-type conformations. Subtle differences in structural dynamics have been identified using hydrogen−deuterium exchange experiments by NMR spectroscopy, suggesting the coexistence of at least two structurally similar but dynamically distinct substates, which undergo slow interconversion on the NMR timescale.

Item Type:Article
Additional Information:Full text available electronically from the School of Pharmacy Library.
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Cancer Research UK Biomolecular Structure Group
ID Code:1341
Journal or Publication Title:Journal of the American Chemical Society
Deposited By:Library Staff
Deposited On:21 Jan 2010 12:23
Last Modified:21 Jan 2010 12:23

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