Delivery of bioactive macromolecules from microporous polymer matrices: release and activity profiles of lysozyme, collagenase and catalase.

Wang, Y., Chang, H-I., Li, X., Alpar, H.O. and Coombes, A. (2009) Delivery of bioactive macromolecules from microporous polymer matrices: release and activity profiles of lysozyme, collagenase and catalase. European Journal of Pharmaceutical Sciences, 37 . pp. 387-394. 10.1016/j.ejps.2009.03.010.

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DOI: 10.1016/j.ejps.2009.03.010

Abstract

Microporous polycaprolactone (PCL) matrices containing lysozyme, collagenase and catalase respectively with molecular weight covering a wide range from 14.3 to 240 kDa were produced by a novel method involving rapid cooling of particle suspensions in dry ice. The enzyme loading efficiency (lysozyme (50%), collagenase (75%) and catalase (90%)) depended on the enzyme molecular weight and the non-solvent used to extract acetone from the hardened matrices. Sustained enzyme release occurred from the PCL matrices over 11 days with retained activity dependent on the particular enzyme used (collagenase 100% activity at 11 days, lysozyme 75–80% at 11 days, catalase 10–20% at 5 days). The present findings confirm the potential of microporous PCL matrices for delivering bioactive macromolecules from implantable/insertable depot-type formulations and tissue engineering scaffolds and recommend catalase as a challenging model protein for evaluating such devices.

Item Type:Article
Additional Information:Full text available electronically from the School of Pharmacy Library.
Uncontrolled Keywords:Polycaprolactone; Tissue engineering; Scaffolds; Enzymes; Matrix device lysozyme; Lysozyme; Collagenase; Catalase; Enzyme activity
Departments, units and centres:Department of Pharmaceutics > Centre for Drug Delivery Research
ID Code:1364
Journal or Publication Title:European Journal of Pharmaceutical Sciences
Deposited By:Library Staff
Deposited On:27 Jan 2010 16:14
Last Modified:17 Nov 2011 17:40

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