Assessment of Cellular Uptake and Cytotoxicity of Carbon Nanotubes Using Flow Cytometry.

Al-Jamal, K.T. and Kostarelos, K. (2010) Assessment of Cellular Uptake and Cytotoxicity of Carbon Nanotubes Using Flow Cytometry. Methods in Molecular Biology (Clifton, N.J.), 625 (3). pp. 123-134. 10.1007/978-1-60761-579-8_11.

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DOI: 10.1007/978-1-60761-579-8_11

Abstract

The field of carbon nanotube (CNT) functionalization is increasingly growing for the purpose of enhancing the biocompatibility of CNT for medical and biological applications. Properties of CNT such as the type of functionalization, charge density, and the dispersibility profile are expected to modulate CNT cellular uptake and toxicity profile in vitro. The assay described here allows for rapid screening of CNT cellular uptake in vitro and assessing the acute cytotoxicity simultaneously. CNT cellular uptake is measured qualitatively by light scattering analysis without differentiating between cell binding and internalisation of the CNT by the cells. In addition, flow cytometry is used to combine light scattering analysis with flow cytometry-based Annexin V/propidium iodide assay to measure the cytotoxicity. This assay is rapid, reliable, and allows for comparative analysis between various types of CNT studied.

Item Type:Article
Uncontrolled Keywords:Carbon nanotubes - CNT - Association - Binding - Internalisation - Toxicity - Flow cytometry - Light scatter - Annexin V/PI - Apoptosis - Necrosis
Departments, units and centres:Department of Pharmaceutics > Centre for Drug Delivery Research
ID Code:1665
Journal or Publication Title:Methods in Molecular Biology (Clifton, N.J.)
Deposited By:Library Staff
Deposited On:14 Jul 2010 11:48
Last Modified:04 May 2012 11:05

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