Evidence for apparent gene instability in the rifamycin-producing oligoketide synthase. Implications for combinatorial biosynthesis and heterologous gene expression

Perić-Concha, N., Castaldo, G. and Long, P.F. (2005) Evidence for apparent gene instability in the rifamycin-producing oligoketide synthase. Implications for combinatorial biosynthesis and heterologous gene expression. Folia Microbiologica, 50 (6). pp. 483-486. 10.1007/BF02931434 .

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DOI: 10.1007/BF02931434

Abstract

The oligoketide (‘polyketide’) synthase leading to the formation of proansamycin X inAmycolatopsis mediterranei also prematurely releases a range of acyclic intermediates from the enzyme complex. We intended to study the chemical biology of this ectopic chain release using RifA as a model protein system; however, we were unable to clone therifA gene in its entirety. Restriction analysis of cosmid clones revealed thatrifA is subject to random deletions at high frequency, especially in central regions of the locus. Examination of the gene sequence in this region reveals a high concentration of inverted repeats; we suggest that these sequences are subject to alteration in secondary structure when cloned outside the environment of theA. mediterranei genome, leading to recombination and deletion.

Item Type:Article
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:1876
Journal or Publication Title:Folia Microbiologica
Deposited By:Library Staff
Deposited On:04 Mar 2011 11:14
Last Modified:04 Nov 2011 10:45

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