Sorbitan monostearate/polysorbate 20 organogels containing niosomes: a delivery vehicle for antigens?

Murdan, S. (1999) Sorbitan monostearate/polysorbate 20 organogels containing niosomes: a delivery vehicle for antigens? European Journal of Pharmaceutical Sciences, 8 (3). p. 177. 10.1016/S0928-0987(99)00014-7.

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DOI: 10.1016/S0928-0987(99)00014-7

Abstract

Multi-component organogels formed using the non-ionic surfactant sorbitan monostearate as gelator have been formulated to contain niosomes. The purpose of this study was to evaluate the potential of these vesicle-in-water-in-oil (v/w/o) gels as delivery vehicles for vaccines. Bovine serum albumin (BSA) and haemagglutinin (HA) were used as model antigens in depot and immunogenicity studies respectively. The complex gels were prepared by the addition of a hot (60°C) aqueous niosome suspension (v/w) to the sol phase (o, an organic solution of the gelator); a vesicle-in-water-in-oil (v/w/o) emulsion was produced which cools to an opaque, semi-solid, thermoreversible v/w/o gel. Light microscopy of the organogel revealed that the microstructure consists of a tubular network of surfactant aggregates in the organic medium, the niosome suspension being dispersed in these surfactant tubules. Therefore, in such gels, the vaccine is thought to be entrapped in the niosomes, themselves located within the sorbitan monostearate tubular network in the organic medium. In vivo, a depot effect was observed following intramuscular administration of the gel containing the entrapped bovine serum albumin, cleared from the injection site over a period of days. The relatively short-lived nature of the depot was thought to arise due to interactions between the gel and the local interstitial fluid which results in gel disintegration in situ. Thus, the niosomes containing antigens are believed to be released from the organic gel. Immunogenicity studies showed that the v/w/o gel as well as one of the controls, the water-in-oil (w/o) gel, possess immunoadjuvant properties and enhance the primary and secondary antibody titres (of total IgG, IgG1, IgG2a and IgG2b) to haemagglutinin antigen. As far as humoral immunity is concerned, the w/o gel showed stronger immunoadjuvant properties compared to the v/w/o gel, being effective at a lower antigen dose i.e 0.1μg HA.

Item Type:Article
Uncontrolled Keywords:Organogels; Niosomes; v/w/o gels; Immunoadjuvant
Departments, units and centres:Department of Pharmaceutics > Department of Pharmaceutics
ID Code:1920
Journal or Publication Title:European Journal of Pharmaceutical Sciences
Deposited By:Library Staff
Deposited On:17 Mar 2011 16:32
Last Modified:17 Mar 2011 16:32

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