The pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines.

Shah, M.M. and Haylett, D.G. (2000) The pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines. British Journal of Pharmacology, 129 (4). pp. 627-630.

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Abstract

The pharmacology of hSK1, a small conductance calcium-activated potassium channel, was studied in mammalian cell lines (HEK293 and COS-7). In these cell types, hSK1 forms an apamin-sensitive channel with an IC(50) for apamin of 8 nM in HEK293 cells and 12 nM in COS-7 cells. The currents in HEK293 cells were also sensitive to tubocurarine (IC(50)=23 microM), dequalinium (IC(50)=0.4 microM), and the novel dequalinium analogue, UCL1848 (IC(50)=1 nM). These results are very different from the pharmacology of hSK1 channels expressed in Xenopus oocytes and suggest the properties of the channel may depend on the expression system. Our findings also raise questions about the role of SK1 channels in generating the apamin-insensitive slow afterhyperpolarization observed in central neurones.

Item Type:Article
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:2044
Journal or Publication Title:British Journal of Pharmacology
Deposited By:Library Staff
Deposited On:05 May 2011 10:35
Last Modified:05 May 2011 10:35

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