Estimation by FACS of the delivery of liposome encapsulated macromolecules into myeloid cells

Alpar, H.O., Bason, A., Hickman, J., Richards, F. and Field, W. (1990) Estimation by FACS of the delivery of liposome encapsulated macromolecules into myeloid cells. International Journal of Pharmaceutics, 62 (2-3). pp. 133-141. 10.1016/0378-5173(90)90227-U.

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DOI: 10.1016/0378-5173(90)90227-U

Abstract

Charged and neutral liposomes were loaded with fluorescein (FITC) labelled albumin. The extent to which the FITC-albumin delivered to non-phagocytic HL-60 cells, zeta potential − 9.8 mV, by the process of fusion, was monitored using both a microscope and flow cytometry (FACS). Positively charged liposomes were most effective in their delivery whilst those with a negative charge showed very little transference ability. The delivery of FITC-albumin from positively charged liposomes was found to be heterogeneous with regard to cell size in the absence of PEG, and more homogeneous in the presence of the fusogen.

Item Type:Article
Uncontrolled Keywords:Liposome; Non-phagocytic cell; Delivery; Macromolecule; Cell surface; Membrane fusion; Fusogen; Flow cytometry
Departments, units and centres:Department of Pharmaceutics > Department of Pharmaceutics
ID Code:2120
Journal or Publication Title:International Journal of Pharmaceutics
Deposited By:Library Staff
Deposited On:20 May 2011 15:16
Last Modified:20 May 2011 15:16

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