Kortenkamp, A. and O'Brien, P. (1994) The generation of DNA single-strand breaks during the reduction of chromate by ascorbic acid and/or glutathione in vitro. Environmental Health Perspectives, 102 Suppl 3 . pp. 237-241.
Full text available as:
|PDF (OPEN ACCESS) - Published Version|
The potential role of iron and copper and the involvement of hydroxyl radicals in the DNA cleavage caused by chromate and glutathione (GSH) has been investigated. We have also studied the ability of chromate, on reaction with ascorbate as well as in mixed solutions of ascorbate and GSH, to cause DNA strand breaks. In both fully demetalated and conventional (i.e., metal contaminated) systems, chromate and GSH induced similar numbers of DNA strand breaks. This observation suggests that traces of iron or copper contaminating the reaction mixtures do not play a major role in the DNA cleavage caused by chromate and GSH. A series of hydroxyl radical scavengers exhibited a protective influence on the induction of DNA strand breaks. However, glucose and sucrose, both strong hydroxyl radical scavengers, showed no concentration-dependent inhibition of DNA cleavage. Competition kinetics studies yielded apparent rate constants that were not consistent with hydroxyl radicals being the species responsible for DNA strand breaks. Ascorbate in combination with chromate was also found to induce strand breaks in DNA; this damage could be attributed to reactive intermediates generated during the reduction. When mixed systems of ascorbate and GSH in the presence of chromate were investigated, there were clearly interactions between the two reductants.
|Departments, units and centres:||Department of Pharmacology > Centre for Toxicology|
|Journal or Publication Title:||Environmental Health Perspectives|
|Deposited By:||Library Staff|
|Deposited On:||23 Jun 2011 15:05|
|Last Modified:||23 Jun 2011 15:06|
Item downloaded times since 23 Jun 2011 15:05.
Repository Staff Only: Item control page
School of Pharmacy Staff Only: Edit a copy to replace this item