Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

Huebner, A., Bratton, D., Whyte, G., Yang, M., deMello, A.J., Abell, C. and Hollfelder, F. (2009) Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. Lab on a Chip, 9 (5). pp. 692-698. 10.1039/b813709a.

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DOI: 10.1039/b813709a

Abstract

We describe the design, fabrication and use of a single-layered poly(dimethylsiloxane) microfluidic structure for the entrapment and release of microdroplets in an array format controlled entirely by liquid flow. Aqueous picoliter droplets are trapped en masse and optically monitored for extended periods of time. Such an array-based approach is used to characterize droplet shrinkage, aggregation of encapsulated E. colicells and enzymatic reactions. We also demonstrate that trapped droplets may be recovered from the microfluidic array for further processing.

Item Type:Article
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2308
Journal or Publication Title:Lab on a Chip
Deposited By:Library Staff
Deposited On:07 Oct 2011 16:12
Last Modified:07 Oct 2011 16:12

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