Bramwell, V.W., Eyles, J.E., Somavarapu, S. and Alpar, H.O. (2002) Liposome/DNA complexes coated with biodegradable PLA improve immune responses to plasmid encoding hepatitis B surface antigen. Immunology, 106 (3). pp. 412-418. 10.1046/j.1365-2567.2002.01448.x.
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We hypothesized that the addition of polymer to the surface of liposome/DNA complexes may potentially enhance in vivo delivery of plasmid DNA to antigen-presenting cells and thereby facilitate enhanced immune responses to encoded protein. BALB/c mice were immunized subcutaneously or intramuscularly three times with a total of 50 µg of the plasmid pRc/CMV-HBs(S) (ayw subtype) encoding for the hepatitis B surface antigen. We measured transgene-specific total immunoglobulin G (IgG), IgG2a, IgG2b and IgG1 antibody responses as well as splenocyte and T-cell proliferation and cytokine production upon re-stimulation following immunization. Modification of lipid/DNA complexes by the polymer precipitation method used here for the addition of poly(d,l-lactic acid) was found to be consistently and significantly more effective than either unmodified liposomal DNA or naked DNA in eliciting transgene-specific immune responses to plasmid-encoded antigen when administered by the subcutaneous route. In addition, the polymer-modified formulations delivered by this route were more effective than naked DNA delivered by the intramuscular route in inducing antibody responses (n=5, P<0·03). Our observations provide ‘proof of principle’ for the use of these multicomponent formulations, which offer potential for manipulation and increased transfection efficiency in vivo for the purposes of genetic immunization.
|Additional Information:||Full text available electronically from the School of Pharmacy Library.|
|Departments, units and centres:||Department of Pharmaceutics > Centre for Drug Delivery Research|
|Journal or Publication Title:||Immunology|
|Deposited By:||Library Staff|
|Deposited On:||31 Aug 2007|
|Last Modified:||18 Nov 2011 09:59|
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