Influence of post-emulsification drying processes on the microencapsulation of Human Serum Albumin

Lane, M.E., Brennan, F. and Corrigan, O.I. (2006) Influence of post-emulsification drying processes on the microencapsulation of Human Serum Albumin. International Journal of Pharmaceutics, 307 (1). pp. 16-22. 10.1016/j.ijpharm.2005.09.026.

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DOI: 10.1016/j.ijpharm.2005.09.026

Abstract

In the present work, methods used to microencapsulate Human Serum Albumin (HSA) in a biodegradable polymer were compared for their effects on the physicochemical characteristics of HSA-loaded microparticles and on the release and integrity of encapsulated HSA. The polymer used was poly(D,L-lactide-co-glycolide) (75:25) (PLGA) (Boehringer Ingelheim, Resomer RG 752, MW 20,900). Microparticles were formulated by (i) w/o/w emulsification and freeze-drying (EFD) or (ii) w/o/w emulsification and spray-drying (ESD). Particle morphology and size were evaluated by scanning electron microscopy and by laser diffraction analysis. Loading, encapsulation efficiency and protein release were determined using a commercial protein assay kit. Protein integrity was evaluated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Particles produced by emulsification/spray-drying exhibited greater diversity in shape than those produced by emulsification/freeze-drying. Additionally, protein loading values were significantly higher for particles produced by emulsification/spray-drying rather than particles produced by emulsification/freeze-drying. The structural integrity of encapsulated protein was confirmed for particles produced by both processes. The fraction of HSA released was similar for both formulations. The emulsification/spray-drying technique described appears to be a rapid and efficient method for the preparation of PLGA microparticles loaded with a model protein

Item Type:Article
Departments, units and centres:Department of Pharmaceutics > Department of Pharmaceutics
ID Code:2395
Journal or Publication Title:International Journal of Pharmaceutics
Deposited By:Library Staff
Deposited On:03 Nov 2011 15:44
Last Modified:03 Nov 2011 15:44

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