Growth cones isolated from developing rat forebrain: uptake and release of GABA and noradrenaline.

Lockerbie, R.O., Gordon-Weeks, P.R. and Pearce, B.R. (1985) Growth cones isolated from developing rat forebrain: uptake and release of GABA and noradrenaline. Brain Research, 353 (2). pp. 265-275. 10.1016/0165-3806(85)90215-9.

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DOI: 10.1016/0165-3806(85)90215-9

Abstract

A growth cone-enriched fraction isolated from neonatal rat forebrain was shown to accumulate gamma-amino [3H]butyric acid ([3H]-GABA) and [3H]noradrenaline ([3H]NA). Uptake of both neurotransmitters was sodium- and temperature-dependent and exhibited saturation kinetics with Km values of 17.7 microM and 4.5 microM respectively and Vmax values of 114 pmol/min/mg protein and 59 pmol/min/mg protein respectively. Electron microscopic autoradiography showed that about 50% of isolated growth cones can accumulate [3H]GABA. Inhibitor studies showed that beta-alanine was a relatively weak inhibitor of [3H]GABA uptake compared to nipecotic acid and diamino-butyric acid. Growth cone fractions preloaded with [3H]GABA and [3H]NA demonstrated a K+ (25 mM) -induced release of both neurotransmitters. Of the K+-stimulated release of [3H]GABA 50% was Ca2+-dependent, whereas the release of [3H]NA was entirely Ca2+-independent.

Item Type:Article
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:2425
Journal or Publication Title:Brain Research
Deposited By:Library Staff
Deposited On:10 Nov 2011 14:04
Last Modified:10 Nov 2011 14:04

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