Uptake and release of [3H]GABA by growth cones isolated from neonatal rat brain

Gordon-Weeks, P.R., Lockerbie, R.O. and Pearce, B.R. (1984) Uptake and release of [3H]GABA by growth cones isolated from neonatal rat brain. Neuroscience Letters, 52 (1-2). pp. 205-210. 10.1016/0304-3940(84)90375-6.

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DOI: 10.1016/0304-3940(84)90375-6


A subcellular fraction highly enriched in neuronal growth cones was isolated from 5-day-old rat forebrain by a recently described method. The growth cone fraction was shown to have a sodium- and temperature-dependent, high-affinity (Km = 4.4 microM) uptake system for [3H]GABA. Electron microscopic autoradiography confirmed that this uptake was into growth cones since only these structures were heavily labelled with silver grains. High potassium induced the release of newly accumulated [3H]GABA from the growth cone fraction, about half of which was Ca2+-dependent. The presence of uptake and release systems for GABA in growth cones may simply reflect the development of growth cones into nerve terminals. Alternatively, these observations may indicate a role for neurotransmitter release in synaptogenesis.

Item Type:Article
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:2426
Journal or Publication Title:Neuroscience Letters
Deposited By:Library Staff
Deposited On:10 Nov 2011 14:08
Last Modified:10 Nov 2011 14:08

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