A simple perfusion chamber for studying neurotransmitter release from cells maintained in monolayer culture

Pearce, B.R., Currie, D.N., Dutton, G.R., Hussey, R.E.G., Beale, R. and Pigott, R. (1981) A simple perfusion chamber for studying neurotransmitter release from cells maintained in monolayer culture. Journal of Neuroscience Methods, 3 (3). pp. 255-259. 10.1016/0165-0270(81)90061-3.

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DOI: 10.1016/0165-0270(81)90061-3

Abstract

A perfusion chamber is described for studying the efflux of putative neurotransmitters from CNS cells maintained in monolayer culture. We have used this apparatus to investigate the efflux of newly accumulated [3H]GABA from cell cultures of the early postnatal rat cerebellum.

Item Type:Article
Uncontrolled Keywords:neurotransmitter release; cell culture; perfusion; GABA efflux
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:2434
Journal or Publication Title:Journal of Neuroscience Methods
Deposited By:Library Staff
Deposited On:10 Nov 2011 14:42
Last Modified:10 Nov 2011 14:42

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