Sayin, B., Somavarapu, S., Li, X.W., Sesardic, D., Senel, S. and Alpar, H.O. (2009) TMC-MCC (N-trimethyl chitosan-mono-N-carboxymethyl chitosan) nanocomplexes for mucosal delivery of vaccines. European Journal of Pharmaceutical Sciences, 38 (4). pp. 362-369. 10.1016/j.ejps.2009.08.010.
Full text not available from this repository.
In this study, for the first time, TMC/MCC complex nanoparticles as a delivery system and as an adjuvant were developed and evaluated to obtain systemic and mucosal immune responses against nasally administered tetanus toxoid (TT). Nanoparticles were developed by complexation between the oppositely charged chitosan derivatives, N-trimethyl chitosan (TMC, polycationic) and mono-N-carboxymethyl chitosan (MCC, polyampholytic) without using any crosslinker for mucosal vaccination. The cellular viability was found to be higher with TMC/MCC complex compared to that of MCC and TMC alone. Size, zeta potential and morphology of the nanoparticles were investigated as a function of preparation method. Nanoparticles with high loading efficacy (95%) and positively charged surface were obtained with an average particle size of 283+/-2.5 nm. The structural integrity of the TT in the nanoparticles was confirmed by SDS-PAGE electrophoresis analysis. Cellular uptake studies indicated that FITC-BSA loaded nanoparticles were effectively taken up into the mouse Balb/c monocyte macrophages. Mice were nasally immunized with TT loaded TMC/MCC complex nanoparticles and compared to that of TMC and MCC nanoparticles. TMC/MCC complex nanoparticles were shown to induce both the mucosal and systemic immune response indicating that this newly developed system has potential for mucosal administration of vaccines.
|Uncontrolled Keywords:||Mucosal immunization; Nanoparticles; Chitosan; Chitosan derivatives; Tetanus toxoid; N-trimethyl chitosan; Mono-N-carboxymethyl chitosan; Nasal vaccination; Nanocomplex|
|Departments, units and centres:||Department of Pharmaceutics > Department of Pharmaceutics|
|Journal or Publication Title:||European Journal of Pharmaceutical Sciences|
|Deposited By:||Library Staff|
|Deposited On:||17 Nov 2011 16:08|
|Last Modified:||17 Nov 2011 16:34|
Item downloaded times since 17 Nov 2011 16:08.
Repository Staff Only: Item control page
School of Pharmacy Staff Only: Edit a copy to replace this item