Immunisation against plague by transcutaneous and intradermal application of subunit antigens

Eyles, J.E., Elvin, S.J., Westwood, A., Lebutt, C.S., Alpar, H.O., Somavarapu, S. and Williamson, E.D. (2004) Immunisation against plague by transcutaneous and intradermal application of subunit antigens. Vaccine, 22 (31-32). p. 4365. 10.1016/j.vaccine.2004.02.049.

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DOI: 10.1016/j.vaccine.2004.02.049

Abstract

We have investigated immunological responses in BALB/c mice following transcutaneous (TC) delivery of fraction 1 (F1) and V subunits from Yersinia pestis in conjunction with an enterotoxin-derived adjuvant (cholera toxin, CT). It was found that two or more TC applications of F1 and V subunits (admixed with cholera toxin) served to elicit significant levels of anti-F1 and V antibodies in the serum of immunised mice. IL-6 secretion from cultured splenocytes derived from immunised mice indicated that a single TC application of F1 and V subunits (admixed with cholera toxin) conferred a cell-mediated response. As compared with intranasal or direct intradermal injection of F1 and V, the numbers of F1/V-specific antibody-forming cells in the spleens of animals immunised by TC application of F1 and V (admixed with CT) was relatively low. It was noted that TC application of F1 and V admixed with CT was very effective for priming responses that were boosted by intranasal or intradermal routes. Similarly, it was found that TC application of F1 and V admixed with CT could be used to efficiently boost pre-existing responses engendered by intradermal injection or intranasal instillation of F1 and V. In order to assess if TC application of F1 and V admixed with CT could protect experimental animals from plague, immunised mice were injected with a virulent strain of Y. pestis. It was found that two TC applications of F1 and V admixed with CT conferred only limited protection against 10(2) MLDs. However, three TC applications of F1 and V admixed with CT conferred solid protection against 10(2) MLDs. Hence we have shown, for the first time, that TC application of F1 and V admixed with CT can protect animals against challenge with a virulent strain of plague causing bacteria. These data suggest that transcutaneous immunisation may be a simple and non-invasive method for immunising individuals against plague.

Item Type:Article
Departments, units and centres:Department of Pharmaceutics > Centre for Drug Delivery Research
ID Code:2465
Journal or Publication Title:Vaccine
Deposited By:Library Staff
Deposited On:17 Nov 2011 16:18
Last Modified:17 Nov 2011 16:18

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