Enhanced properties of discrete pulmonary deoxyribonuclease I (DNaseI) loaded PLGA nanoparticles during encapsulation and activity determination

Osman, R., Kan, P.L., Awad, G., Mortada, N., EL-Shamy, A-E. and Alpar, H.O. (2011) Enhanced properties of discrete pulmonary deoxyribonuclease I (DNaseI) loaded PLGA nanoparticles during encapsulation and activity determination. International Journal of Pharmaceutics, 408 (1-2). pp. 257-265. 10.1016/j.ijpharm.2011.02.013.

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DOI: 10.1016/j.ijpharm.2011.02.013

Abstract

In the present work, DNaseI loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) for pulmonary delivery were prepared using emulsion solvent evaporation. The effects of the various formulation and experimental variables on the size and morphological characteristics of the particles as well as on the encapsulation efficiency were investigated. The stability of the encapsulated DNaseI was evaluated and the respirable fraction was determined. Cytotoxicity of the NPs was evaluated on lung epithelial cells. The results showed that by using leucine and dipalmito-phosphatidyl-choline (DPPC), discrete NPs with 76% retained biological activity were prepared. A high respirable fraction (particles below 6 μm) reaching 71.3% was achieved after nebulization of the NP suspension. The results revealed the suitability of the prepared particles for pulmonary delivery and highlighted the role of excipients in the stabilization of DNaseI against the stresses encountered during preparation

Item Type:Article
Uncontrolled Keywords:Pulmonary; DNaseI; Nanoparticles; Emulsion-solvent evaporation; Radial enzyme diffusion; Leucine; DPPC
Departments, units and centres:Department of Pharmaceutics > Centre for Drug Delivery Research
ID Code:2466
Journal or Publication Title:International Journal of Pharmaceutics
Deposited By:Library Staff
Deposited On:17 Nov 2011 16:53
Last Modified:17 Nov 2011 16:53

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