Shannon, K.P., Fung, K-W., Stapleton, P.D., Anthony, R., Power, E. and French, G.L. (1998) A hospital outbreak of extended-spectrum β-lactamase-producing Klebsiella pneumoniae investigated by RAPD typing and analysis of the genetics and mechanisms of resistance. Journal of Hospital Infection, 39 (4). pp. 291-300. 10.1016/S0195-6701(98)90294-8.
Full text not available from this repository.
Between July and September 1997 a ceftazidime- and aminoglycoside-resistant strain of Klebsiella pneumoniae infected or colonized seven patients on three paediatric wards at Guy's Hospital in London. The patients were mostly neonates or infants recovering from cardiac surgery for congenital defects. The organism was probably introduced by an asymptomatic patient from Greece and the subsequent outbreak could largely be explained by person-to-person spread on individual wards and frequent transfers of patients between wards. The outbreak was controlled by patient isolation and attention to handwashing, and there were no fatalities. The organisms were non-typeable by serology but had a characteristic RAPD profile. They produced the extended-spectrum β-lactamase SHV-5 and the aminoglycoside-modifying enzymes AAC(6′) + probably AAC(3)II, encoded on a conjugative plasmid of approximately 160 kb. Two other patients had multi-resistant klebsiellas, one of them an SHV-5 producer and one a TEM-5 producer, but these could be distinguished from each other and from the outbreak strain by serological and RAPD typing and by the genetics and mechanisms of their resistances. Three other multi-resistant enterobacteria were isolated during the outbreak: an Escherichia coli that had acquired the 160 kb resistance plasmid from the epidemic klebsiella, a Citrobacter isolated from one of the patients with the klebsiella but which did not produce SHV-5, and a TEM-5-producing Enterobacter. This outbreak illustrates the importance of screening patients from high-risk areas for multiply-resistant organisms on admission, and the value of bacterial typing and analysis of resistance mechanisms to define the epidemiology of hospital infection.
|Uncontrolled Keywords:||lebsiella pneumonia; SHV-5 extended-spectrum β-lactamase; paediatrics; outbreak; RAPD typing; plasmid; transferable resistance|
|Departments, units and centres:||Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry|
|Journal or Publication Title:||Journal of Hospital Infection|
|Deposited By:||Library Staff|
|Deposited On:||18 Nov 2011 15:54|
|Last Modified:||18 Nov 2011 15:54|
Item downloaded times since 18 Nov 2011 15:54.
Repository Staff Only: Item control page
School of Pharmacy Staff Only: Edit a copy to replace this item