Immunohistochemical localization of N-methyl-?-aspartate receptor subunits in the adult murine hippocampal formation: evidence for a unique role of the NR2D subunit

Thompson, C.L., Drewerya, D.L., Atkins, H.D., Stephenson, F.A. and Chazot, P.L. (2002) Immunohistochemical localization of N-methyl-?-aspartate receptor subunits in the adult murine hippocampal formation: evidence for a unique role of the NR2D subunit. Molecular Brain Research, 102 (1-2). pp. 55-61. 10.1016/S0169-328X(02)00183-3.

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DOI: 10.1016/S0169-328X(02)00183-3

Abstract

NMDA receptors were immunopurified from adult mouse forebrain and screened by immunoblotting. NR1 was co-associated with NR2A, NR2B and NR2D but not NR2C, nor was NR2C detected in adult mouse hippocampal membranes. The anatomical distribution of NR1, 2A, 2B and 2D was mapped in the adult murine hippocampal formation. NR1-like immunoreactivity was localised to cell bodies of pyramidal neurons, granule cells and hilar cells of the dentate gyrus. Apical dendrites of the CA subfields and hilar cells were also immunopositive. NR2A- and NR2B-like immunoreactivity essentially co-localised with that of NR1 implying co-assembly of all three subunits in this brain structure. NR2D-like immunoreactivity was distinct, being totally excluded from pyramidal, granule and hilar cell bodies. Strong, punctate staining was restricted to the oriens layer of CA1 and the stratum lucidum of CA3 consistent with labelling of presynaptic receptors. Less intense staining was also observed in the internal third of the molecular layer of the dentate gyrus.

Item Type:Article
Uncontrolled Keywords:Neurotransmitters, modulators, transporters, and receptors, Excitatory amino acid receptors: structure, function and expression
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2501
Journal or Publication Title:Molecular Brain Research
Deposited By:Library Staff
Deposited On:24 Nov 2011 12:39
Last Modified:24 Nov 2011 12:39

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