A quantitative study of the effects of some muscarinic antagonists on the guinea-pig olfactory cortex slice.

Williams, S.H. and Constanti, A. (1988) A quantitative study of the effects of some muscarinic antagonists on the guinea-pig olfactory cortex slice. British Journal of Pharmacology, 93 (4). pp. 855-862.

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Official URL: http://www.ukpmc.ac.uk/articles/PMC1853873

Abstract

1. Muscarinic depression of the electrically-evoked surface-negative field potential (N-wave) was measured in guinea-pig olfactory cortex slices maintained in vitro. 2. The effects of three muscarinic receptor antagonists, pirenzepine, atropine and gallamine on this muscarinic response were analysed in detail. 3. Pirenzepine was a potent competitive antagonist of carbachol (CCh)-evoked responses. Schild plot analysis yielded a pA2 value of 7.9 (Schild slope constrained to unity). A similar analysis for atropine versus CCh responses gave a pA2 of 8.9. 4. Combination experiments using pirenzepine and atropine produced dose-ratio shifts close to those expected for two antagonists competing for a similar receptor site. 5. Gallamine was only a weak antagonist of responses to CCh. 6. Oxotremorine behaved as a competitive antagonist at this muscarinic receptor (pA2 = 6.1). 7. It is concluded that the presynaptic muscarinic receptor mediating depression of the N-wave in the olfactory cortex slice is of the M1-subtype.

Item Type:Article
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:2536
Journal or Publication Title:British Journal of Pharmacology
Deposited By:Library Staff
Deposited On:25 Nov 2011 10:19
Last Modified:25 Nov 2011 10:19

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