Immunohistochemical localization of N-methyl-D-aspartate receptor NR1, NR2A, NR2B and NR2C/D subunits in the adult mammalian cerebellum.

Thompson, C.L., Drewerya, D.L., Atkins, H.D., Stephenson, F.A. and Chazot, P.L. (2000) Immunohistochemical localization of N-methyl-D-aspartate receptor NR1, NR2A, NR2B and NR2C/D subunits in the adult mammalian cerebellum. Neuroscience Letters, 283 (2). pp. 85-88. 10.1016/S0304-3940(00)00930-7 .

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DOI: 10.1016/S0304-3940(00)00930-7

Abstract

The distributions of the N-methyl-D-aspartate (NMDA) receptor NR1, NR2A, NR2B and NR2C/D subunits were mapped in adult mouse cerebellum using subunit-specific antibodies. Immunostaining with anti-NR1 antibodies was prominent in cell bodies and dendritic arbors of Purkinje cells, was light to moderate in cerebellar granule cells, Golgi interneurons and interneurons in the molecular layer. Anti-NR2A subunit-specific antibody staining of mouse cerebellum was moderate in the granule cells, and moderate to dense in Purkinje neurons and Bergmann glia. However, Purkinje neurons were not immunolabelled in adult rat brain. Anti-NR2B subunit-specific immunostaining was prominent in Purkinje cell bodies and dendrites but absent from the granule cell layer. Anti-NR2C/D subunit-specific immunostaining was largely restricted to cerebellar granule cells. These studies reveal that NMDA receptor subunits display distinct but overlapping expression patterns in the adult mammalian cerebellum. Furthermore, we have observed some differences between rats and mice in terms of the NMDA receptor subunits expressed in specific cerebellar cell types.

Item Type:Article
Uncontrolled Keywords:N-methyl-d-aspartate receptor; Cerebellum; Mammalian central nervous system; Immunohistochemistry; NR1 subunit; NR2A subunit; NR2B subunit; NR2C/D subunit; Subunit-specific antibodies
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2543
Journal or Publication Title:Neuroscience Letters
Deposited By:Library Staff
Deposited On:25 Nov 2011 11:16
Last Modified:25 Nov 2011 11:16

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