Bidirectional regulation of GABAA receptor alpha1 and alpha6 subunit expression by a cyclic AMP-mediated signalling mechanism in cerebellar granule cells in primary culture.

Thompson, C.L., Pollard, S. and Stephenson, F.A. (1996) Bidirectional regulation of GABAA receptor alpha1 and alpha6 subunit expression by a cyclic AMP-mediated signalling mechanism in cerebellar granule cells in primary culture. Journal of Neurochemistry, 67 (1). pp. 434-437.

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Abstract

Forskolin treatment of cerebellar granule cells in culture resulted in bidirectional regulation of the expression of GABAA receptor alpha1 and alpha6 subunits. Thus, forskolin applied at 2 days in vitro (DIV) increased expression of the alpha1 subunit but decreased the expression of the alpha6 subunit. Values with respect to control cultures, both assayed at 9 DIV by immunoblotting, were 310 +/- 48% for alpha1 and 25 +/- 16% for the alpha6 subunit. Similar effects were evoked following chronic treatment with both dibutyryl cyclic AMP and 3-isobutyl-1-methylxanthine. Dideoxyforskolin had no effect on the level of expression of either the alpha1 or the alpha6 GABAA receptor subunits. The changes in subunit expression were accompanied by a 1.7-fold increase in number of total specific [3H]Ro 15-4513 binding sites expressed by intact cerebellar granule cells. This increase in total binding sites was accommodated by a 2.7-fold increase in number of diazepam-sensitive Ro 15-4513 binding sites in accordance with the observed increase in alpha1 subunit expression. The number of diazepam-insensitive subtype of binding sites were not significantly changed. These results suggest that GABAA receptor subtype expression can be differentially regulated by intracellular cyclic AMP concentration.

Item Type:Article
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2560
Journal or Publication Title:Journal of Neurochemistry
Deposited By:Library Staff
Deposited On:25 Nov 2011 14:22
Last Modified:25 Nov 2011 14:22

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