Identification of the GABAA receptor alpha 3 subunit in the IMR-32 neuroblastoma cell line.

Noble, P.J., Anderson, S.M., De Souza, R.J., Cross, A.J. and Stephenson, F.A. (1993) Identification of the GABAA receptor alpha 3 subunit in the IMR-32 neuroblastoma cell line. Journal of Neurochemistry, 61 (2). pp. 752-725.

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Abstract

A previous report has described the presence of t-[35S]-butylbicyclophosphorothionate binding sites and GABA-gated Cl- flux in the human neuroblastoma IMR-32 cell line. We now report the further characterisation of this binding site and, even more important, the identification of the GABAA receptor alpha 3 sub-unit expressed in these cells. Cell membranes prepared from IMR-32 cells were screened by immunoblotting for reactivity with various GABAA receptor alpha subunit-specific antibodies. Of these, only anti-Cys alpha 3 454-467 antibodies recognised specifically and in a dose-dependent manner an immunoreactive band. This M(r) 58,000 immunoreactive species and the N-deglycosylated derivatives were both coincident with the respective homologues found in both calf cerebral cortex membranes and purified receptor preparations. This is the first report of the identification of a specific GABAA receptor subunit expressed in a human cell line, and it therefore provides a convenient model for the study of receptor structure and regulation.

Item Type:Article
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2572
Journal or Publication Title:Journal of Neurochemistry
Deposited By:Library Staff
Deposited On:25 Nov 2011 15:40
Last Modified:25 Nov 2011 15:40

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