Immunological detection of the NMDAR1 glutamate receptor subunit expressed in embryonic kidney 293 cells and in rat brain.

Chazot, P.L., Cik, M. and Stephenson, F.A. (1992) Immunological detection of the NMDAR1 glutamate receptor subunit expressed in embryonic kidney 293 cells and in rat brain. Journal of Neurochemistry, 59 (3). pp. 1176-1178. 10.1111/j.1471-4159.1992.tb08364.x.

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DOI: 10.1111/j.1471-4159.1992.tb08364.x

Abstract

The rat NMDAR1 (N-methyl-D-aspartate receptor) was expressed transiently in human embryonic kidney cells. Transfected cell homogenates showed saturable [3H]MK-801 binding activity that was best fit by a single high-affinity site with a KD of 9 nM and a Bmax of 113 fmol of binding sites/mg of protein. Antibodies raised against the peptide sequence NMDAR1 (929-938) coupled to keyhole limpet haemocyanin specifically recognised a single band with M(r) 117,000 in immunoblots from adult rat brain. In the transfected cells, the antibody recognised two bands: one with M(r) 117,000, which was coincident with that from brain membranes, and one with M(r) 97,000, which was identified as nonglycosylated NMDAR1 subunit. These results identify the NMDAR1 of rat brain and further show that the homooligomer binds MK-801, albeit at low efficiency.

Item Type:Article
Uncontrolled Keywords:N-Methyl-d-aspartate;Glutamate;Receptor;Antibody;Glycosylation
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2577
Journal or Publication Title:Journal of Neurochemistry
Deposited By:Library Staff
Deposited On:25 Nov 2011 15:59
Last Modified:25 Nov 2011 15:59

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