Rosado, H., O’Neill, A.J., Blake, K., Walther, M., Long, P.F., Hinds, J. and Taylor, P.W. (2011) Rotating wall vessel alters protein secretion and global gene expression by Staphylococcus aureus. International Journal of Astrobiology, 11 (2). pp. 71-81.
Full text not available from this repository.
Staphylococcus aureus is routinely recovered from air and surface samples taken aboard the International Space Station (ISS) and poses a health threat to crew. As bacteria respond to the low shear forces engendered by continuous rotation conditions in a Rotating Wall Vessel (RWV) and the reduced gravitational field of near-Earth flight by altering gene expression, we examined the effect of low-shear RWV growth on protein secretion and gene expression by three S. aureus isolates. When cultured under 1 g, the total amount of protein secreted by these strains varied up to fourfold; under continuous rotation conditions, protein secretion by all three strains was significantly reduced. Concentrations of individual proteins were differentially reduced and no evidence was found for increased lysis. These data suggest that growth under continuous rotation conditions reduces synthesis or secretion of proteins. A limited number of changes in gene expression under continuous rotation conditions were noted: in all isolates vraX, a gene encoding a polypeptide associated with cell wall stress, was down-regulated. A vraX deletion mutant of S. aureus SH1000 was constructed: no differences were found between SH1000 and ΔvraX with respect to colony phenotype, viability, protein export, antibiotic susceptibility, vancomycin kill kinetics, susceptibility to cold or heat and gene modulation. An ab initio protein-ligand docking simulation suggests a major binding site for β-lactam drugs such as imipenem. If such changes to the bacterial phenotype occur during spaceflight, they will compromise the capacity of staphylococci to cause systemic infection and to circumvent antibacterial chemotherapy.
|Uncontrolled Keywords:||bacterial microarray, continuous rotation conditions, exoproteome, Staphylococcus aureus, vraX|
|Departments, units and centres:||Department of Pharmaceutics > Department of Pharmaceutics|
|Journal or Publication Title:||International Journal of Astrobiology|
|Deposited By:||Library Staff|
|Deposited On:||02 Dec 2011 17:30|
|Last Modified:||26 Apr 2012 17:52|
Item downloaded times since 02 Dec 2011 17:30.
Repository Staff Only: Item control page
School of Pharmacy Staff Only: Edit a copy to replace this item