Hehner, S.P., Heinrich, M., Bork, P.M., Vogt, M., Ratter, F., Lehmann, V., Schulze-Osthoff, K., Dröge, W. and Schmitz, M.L. (1998) Sesquiterpene Lactones Specifically Inhibit Activation of NF-kappa B by Preventing the Degradation of Ikappa B-alpha and Ikappa B-beta Hehner, S.P.a , Heinrich, M.b , Bork, P.M.b , Vogt, M.c , Ratter, F.a , Lehmann, V.a , Schulze-Osthoff, K.c , Dröge, W.a , Schmitz, M.L. Journal of Biological Chemistry, 273 (3). pp. 1288-1297. 10.1074/jbc.273.3.1288.
Full text not available from this repository.
Extracts from certain Mexican Indian medicinal plants used in traditional indigenous medicine for the treatment of inflammations contain sequiterpene lactones (SLs), which specifically inhibit the transcription factor NF-κB (Bork, P.M., Schmitz, M. L., Kuhnt, M., Escher, C., and Heinrich, M. (1997) FEBS Lett. 402, 85-90). Here we show that SLs prevented the activation of NF-κB by different stimuli such as phorbol esters, tumor necrosis factor-α, ligation of the T-cell receptor, and hydrogen peroxide in various cell types. Treatment of cells with SLs prevented the induced degradation of IκB-α and IκB-β by all these stimuli, suggesting that they interfere with a rather common step in the activation of NF-κB. SLs did neither interfere with DNA binding activity of activated NF-κB nor with the activity of the protein tyrosine kinases p59(fyn) and p60(src). Micromolar amounts of SLs prevented the induced expression of the NF-κB target gent intracellular adhesion molecule 1. Inhibition of NF-κB by SLs resulted in an enhanced cell killing of murine fibroblast cells by tumor necrosis factor- α. Sis lacking an exomethylene group in conjugation with the lactone function displayed no inhibitory activity on NF-κB. The analysis of the cellular redox state by fluorescence-activated cell sorter showed that the SLs had no direct or indirect anti-oxidant properties.
|Departments, units and centres:||Department of Pharmaceutical and Biological Chemistry > Centre for Pharmacognosy and Phytotherapy|
|Journal or Publication Title:||Journal of Biological Chemistry|
|Deposited By:||Library Staff|
|Deposited On:||19 Jan 2012 15:04|
|Last Modified:||19 Jan 2012 15:04|
Item downloaded times since 19 Jan 2012 15:04.
View statistics for "Sesquiterpene Lactones Specifically Inhibit Activation of NF-kappa B by Preventing the Degradation of Ikappa B-alpha and Ikappa B-beta Hehner, S.P.a , Heinrich, M.b , Bork, P.M.b , Vogt, M.c , Ratter, F.a , Lehmann, V.a , Schulze-Osthoff, K.c , Dröge, W.a , Schmitz, M.L."
Repository Staff Only: Item control page
School of Pharmacy Staff Only: Edit a copy to replace this item