The use of near-infrared spectroscopy for the determination of eugenol content in clove oils

Wilson, N.D., Watt, R.A. and Moffat, A.C. (2001) The use of near-infrared spectroscopy for the determination of eugenol content in clove oils. AAPS Pharmaceutical Sciences, 3 (Supplement 630).

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Purpose. Near-Infrared (NIR) spectroscopy is an ideal technique for the characterisation of natural materials used in traditional and complementary medicine as it requires no sample preparation and the spectra obtained represent the sample as a whole. It has also been shown previously that NIR spectroscopy can be used to determine the Cineole content of Eucalyptus oil. Carrying on from this study we undertook work to determine if it was possible to quantify chemical constituents in other essential oils. The work presented here concerns the development of a rapid and accurate calibration for the determination of Eugenol content in Clove oil. Methods. 25 samples of Clove oil were scanned on the FOSS NIRSystems 6500 spectrophotometer with a Rapid Content Sampler module and Vision software version 2.20. Five spectra were obtained for each oil, which were then averaged for use in the development of a calibration equation. The Eugenol content of the oils was determined using an HPLC method and these reference values were assigned to the corresponding spectrum for the oils. Sesame software (Bran & Luebbe) was used for the data analysis. The spectra were converted into 2nd derivative form and a Multiple Linear Regression method was employed over the whole wavelength range (1100nm to 2500nm). The best two-wavelength combination for which the Absorbance values had the highest correlation to the reference data was selected. 20 samples were assigned to the calibration set and 5 samples to the validation set. Results. The multiple correlation coefficient for the calibration obtained was 0.98 and the Standard Error (% m/m Eugenol) for the calibration and validation sets was 1.10 and 1.53 respectively. Conclusions. The results suggest that Near-Infrared spectroscopy would be a suitable method for the rapid and accurate determination of the Eugenol content of Clove oils.

Item Type:Article
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Centre for Cancer Medicine
ID Code:283
Journal or Publication Title:AAPS Pharmaceutical Sciences
Deposited By:Library Staff
Deposited On:19 Oct 2006
Last Modified:19 Sep 2007 11:13

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