Bayele, H.K., Ramaswamy, C., Wilderspin, A.F., Srai, K.S., Toth, I. and Florence, A.T. (2006) Protein transduction by lipidic peptide dendrimers. Journal of Pharmaceutical Sciences, 95 (6). pp. 1227-1237. 10.1002/jps.20606.
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We investigated the potential of a new family of lipidic peptide dendrimers in protein transduction into cultured cells. Dendrimer-protein interaction was determined by gel retardation assays using purified recombinant protein. To assess intracellular protein delivery, two marker proteins were used: recombinant firefly luciferase and a Cy3-labeled monoclonal antibody to the c-myc proto-oncogene. Protein delivery was determined by luciferase assays and fluorescence microscopy, respectively. While there was minimal delivery of luciferase or antibody in the absence of the dendrimers, the latter increased protein delivery substantially. Luciferase delivery was concentration and cell type-dependent; the efficiency of delivery also varied with the number of terminal amino groups on the dendrimers. In previous reports, we showed that these dendrimers could be used for gene and drug delivery; the data we report herein suggest that they may also be capable of intracellular protein delivery. This finding has important implications for the use of these dendrimers in protein therapeutics and vaccinology.
|Uncontrolled Keywords:||Antibody; Lipidic; Luciferase; Peptide dendrimer; Protein delivery; Vector|
|Departments, units and centres:||Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry|
|Journal or Publication Title:||Journal of Pharmaceutical Sciences|
|Deposited By:||Library Staff|
|Deposited On:||24 Feb 2012 17:52|
|Last Modified:||24 Feb 2012 17:52|
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