DNA transfection and transfected cell viability using amphipathic asymmetric dendrimers

Shah, D.S., Sakthivel, T., Toth, I., Florence, A.T. and Wilderspin, A.F. (2000) DNA transfection and transfected cell viability using amphipathic asymmetric dendrimers. International Journal of Pharmaceutics, 208 (1-2). pp. 41-48. 10.1016/S0378-5173(00)00534-2.

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DOI: 10.1016/S0378-5173(00)00534-2

Abstract

Amphipathic asymmetric dendrimers have been investigated for use in delivery of genes into cells, with the objective of optimising transfection efficiency and maintaining cell viability. We have synthesised amphipathic asymmetric dendrimers by solid phase methods. The ability of two of these to transfect BHK cells in culture with β-galactosidase gene was determined by X-gal staining. Cell viability was measured by the MTT assay for BHK cells, and by spectroscopy for lysis of erythrocytes. Interactions between dendrimer and DNA were investigated by agarose gel electrophoresis. BHK cells were optimally transfected at 5:1 +/- charge ratio yielding 20% cells receiving at least one copy of the plasmid. Cell viability decreased when the dendrimer to DNA ratio exceeded 5:1. Raising the pH significantly affected the electrophoretic mobility of complexes of dendrimer and DNA. We conclude that amphipathic asymmetric dendrimers enable efficient plasmid DNA uptake into BHK cells. Cell viability is maintained at high concentrations of dendrimer when complexed with DNA at a 5:1 +/- charge ratio. Efficiency of transfection and cell viability suggest the system may be suitable for gene delivery in vivo

Item Type:Article
Uncontrolled Keywords:Dendrimers; Gene delivery; Gene expression; Non-viral vectors; Transfection
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2877
Journal or Publication Title:International Journal of Pharmaceutics
Deposited By:Library Staff
Deposited On:01 Mar 2012 10:07
Last Modified:01 Mar 2012 10:07

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