Novel cationic lipidic peptide dendrimer vectors in vitro gene delivery

Toth, I., Sakthivel, T., Wilderspin, A.F., Bayele, H., O'Donnell, M., Perry, K., Pasi, K.J., Lee, C.A. and Florence, A.T. (1999) Novel cationic lipidic peptide dendrimer vectors in vitro gene delivery. STP Pharma Sciences, 9 (1). pp. 93-99.

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Abstract

Cationic lipidic dendrimers with a well-defined diameter and a precise number of terminal amines (from eight to thirty-two groups) were synthesised using a solid support. The application of dendrimers with widely varied geometries in gene delivery has been studied by estimating transfection efficiency of members of the series, with variable branch length, position of attachment of lipid, the presence of a sugar unit and presence of a nuclear localization signal peptide. The transfection activity of the products was assayed in vitro on Cos-7 (fibroblast) cells. Dendrimers (1a and 1b) displayed high transfection activities. Results indicated that the presence of more amino groups on the surface of the dendrimers could enhance gene delivery. A primary physicochemical characterization of the DNA/lipid complexes demonstrated the minimum amount of dendrimer required for the transfection of 2.5 lag plasmid (10 μg/ml for the dendrimers with eight free amino terminals and 5 and 2.5 μg/ml for the dendrimers with sixteen and thirty-two free amino terminals, respectively).

Item Type:Article
Uncontrolled Keywords:Cationic lipids; Dendrimers; Gene delivery; Lipidic polyamide dendrimers; Non-viral vectors; Transfection
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2878
Journal or Publication Title:STP Pharma Sciences
Deposited By:Library Staff
Deposited On:01 Mar 2012 10:16
Last Modified:01 Mar 2012 10:16

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