Effect of free versus liposomal-complexed pentamidine isethionate on biological characteristics of Acanthamoeba castellanii in vitro

Siddiqui, R., Syed, A.F., Tomas, S., Prieto-Garcia, J.M. and Khan, N.A. (2009) Effect of free versus liposomal-complexed pentamidine isethionate on biological characteristics of Acanthamoeba castellanii in vitro. Journal of Medical Microbiology, 58 (3). pp. 327-330.

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Abstract

Acanthamoeba is an opportunistic protozoan pathogen that can cause blinding keratitis and a rare but fatal encephalitis involving the central nervous system with a very poor prognosis. This is due to limited availability of effective anti-acanthamoebic drugs. Here, we tested whether the use of liposomes can improve the potency of pentamidine isethionate, an anti-amoebic compound. The liposomes consisted of L-α-phosphatidylcholine and cholesterol or ergosterol in a molar ratio of 1 :5. Pentamidine isethionate was incorporated to achieve a final drug to lipid ratio of 1 :5. At a drug concentration of 10 μgm-1, the liposomal drug was >12 times more effective than the free drug at preventing Acanthamoeba binding to human cells and significantly more effective in reducing parasite-mediated human cell cytopathogenicity, compared with the drug alone. Both the free and liposomal drug blocked Acanthamoeba encystation.

Item Type:Article
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:2984
Journal or Publication Title:Journal of Medical Microbiology
Deposited By:Library Staff
Deposited On:16 Mar 2012 10:28
Last Modified:16 Mar 2012 12:47

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