Siddiqui, R., Syed, A.F., Tomas, S., Prieto-Garcia, J.M. and Khan, N.A. (2009) Effect of free versus liposomal-complexed pentamidine isethionate on biological characteristics of Acanthamoeba castellanii in vitro. Journal of Medical Microbiology, 58 (3). pp. 327-330.
Full text not available from this repository.
Acanthamoeba is an opportunistic protozoan pathogen that can cause blinding keratitis and a rare but fatal encephalitis involving the central nervous system with a very poor prognosis. This is due to limited availability of effective anti-acanthamoebic drugs. Here, we tested whether the use of liposomes can improve the potency of pentamidine isethionate, an anti-amoebic compound. The liposomes consisted of L-α-phosphatidylcholine and cholesterol or ergosterol in a molar ratio of 1 :5. Pentamidine isethionate was incorporated to achieve a final drug to lipid ratio of 1 :5. At a drug concentration of 10 μgm-1, the liposomal drug was >12 times more effective than the free drug at preventing Acanthamoeba binding to human cells and significantly more effective in reducing parasite-mediated human cell cytopathogenicity, compared with the drug alone. Both the free and liposomal drug blocked Acanthamoeba encystation.
|Departments, units and centres:||Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry|
|Journal or Publication Title:||Journal of Medical Microbiology|
|Deposited By:||Library Staff|
|Deposited On:||16 Mar 2012 10:28|
|Last Modified:||16 Mar 2012 12:47|
Item downloaded times since 16 Mar 2012 10:28.
Repository Staff Only: Item control page
School of Pharmacy Staff Only: Edit a copy to replace this item