NMR lipid profile of Agaricus bisporus

Bonzom, P.M., Nicolaou, A., Zloh, M., Baldeo, W. and Gibbons, W.A. (1999) NMR lipid profile of Agaricus bisporus. Phytochemistry, 50 (8). pp. 1311-1321. 10.1016/S0031-9422(98)00703-1.

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DOI: 10.1016/S0031-9422(98)00703-1

Abstract

Lipids extracted from freeze dried and powdered cultivated Agaricus bisporus by the Bligh and Dwyer method, were subjected to 1D-proton and 2D- COSY NMR analysis. The diacylglycerophospholipids, mono-, di- and tri- glycerides, ether lipids, sphingolipids and steroidal lipids were studied qualitatively and quantitatively. Our findings suggested that (a) ethanolamines and cholines were the predominant diacylphospholipids, (b) sterols, mainly ergosterol, were present in relatively large quantities and (c) and phospholipid fatty acid composition consisted almost exclusively of linoleic acid. This type of detailed data on lipid composition was accurately and rapidly obtained in one step, without chemical modification of the sample. Additional information on four classes of lipid, including their fatty acid composition was obtained after separating the total lipid extract by NH2-aminopropyl Certify II Bond Elut solid phase chromatography and analysing the NMR spectra of each class of lipids. The results demonstrated the potential of the method for the study of plant metabolism, development and taxonomy.

Item Type:Article
Uncontrolled Keywords:Agaricus bisporus; Basidiomycetes; Lipids; Mushroom; NMR
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry
ID Code:3050
Journal or Publication Title:Phytochemistry
Deposited By:Library Staff
Deposited On:23 Mar 2012 10:18
Last Modified:23 Mar 2012 10:18

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