Arginine release from rat cerebellar astrocytes: autocrine roles for glutamate and nitric oxide?

Segieth, J., Fowler, L.J., Whitton, P.S. and Pearce, B.R. (2004) Arginine release from rat cerebellar astrocytes: autocrine roles for glutamate and nitric oxide? Neuroscience Letters, 372 (3). pp. 262-265. 10.1016/j.neulet.2004.09.049.

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DOI: 10.1016/j.neulet.2004.09.049


In this study we have investigated the relationship between glutamate and arginine release from cultured cerebellar astrocytes. We found that the nitric oxide (NO) donor S-nitroso-N-acetylpenicillamine (SNAP) promoted the release of both amino acids in a concentration-dependent manner, and that these responses were partially reversed by a guanylate cyclase inhibitor. Application of the non-NMDA glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) resulted in a 60% reduction in basal arginine release but no change in that of glutamate. This effect was not overcome by the subsequent addition of SNAP despite a two-fold increase in glutamate release. Incubation with the nitric oxide synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME) elicited 40 and 60% reductions in the basal release of glutamate and arginine, respectively. Basal release of both amino acids was restored by the addition of SNAP. We conclude that glutamate released from cerebellar astrocytes in response to increased levels of extracellular NO acts in an autocrine manner to promote arginine release via the activation of non-NMDA receptors. In addition, our data suggest that basal glutamate release is regulated to some extent by tonic NO synthesis in these cells.

Item Type:Article
Uncontrolled Keywords:Glutamate; Arginine; Nitric oxide; Cerebellar astrocytes
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:3117
Journal or Publication Title:Neuroscience Letters
Deposited By:Library Staff
Deposited On:27 Apr 2012 12:28
Last Modified:27 Apr 2012 12:28

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