Shotgun Cloning and Heterologous Expression of the Patellamide Gene Cluster as a Strategy to Achieving Sustained Metabolite Production

Long, P.F., Dunlap, W.C., Battershill, C,.N. and Jaspars, M. (2005) Shotgun Cloning and Heterologous Expression of the Patellamide Gene Cluster as a Strategy to Achieving Sustained Metabolite Production. ChemBioChem, 6 (10). pp. 1760-1765. 10.1002/cbic.200500210.

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DOI: 10.1002/cbic.200500210

Abstract

Shotgun cloning into E. coli of genomic DNA from Prochloron sp., symbiont of the seasquirt Lissoclinum patella, resulted in the heterologous expression of the patellamide gene cluster and subsequent production of patellamide D (1) and ascidiacyclamide (2) at levels of 80–100 ng mL−1.

Item Type:Article
Uncontrolled Keywords:peptides;Escherichia coli;heterologous expression;HPLC-MS;natural products;patellamides
Departments, units and centres:Department of Pharmaceutics > Department of Pharmaceutics
ID Code:3215
Journal or Publication Title:ChemBioChem
Deposited By:Library Staff
Deposited On:11 May 2012 17:24
Last Modified:11 May 2012 17:24

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