Castaldo, G., Crosby, J. and Long, P.F. (2005) Exploring protein interactions on a minimal type II polyketide synthase using a yeast two-hybrid system. Food Technology and Biotechnology , 43 (2). pp. 109-112.
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Interactions between proteins that form the 'minimal' type II polyketide synthase in the doxorubicin producing biosynthetic pathway from Streptomyces peucetius were investigated using a yeast two-hybrid system (Y2H). Proteins that function as the so called 'chain length factor' (DpsB) and putative transacylase (DpsD) were found to interact with the ketosynthase subunit (DpsA), which can also interact with itself. On the basis of these results we propose a head-to-tail homodimeric structure, which is consistent with previously published in vivo mutagenesis studies. No interactions were found between the acyl-carrier protein (DpsG) and any of the other constituents of the complex, however, transient interactions, not detectable using the Y2H system, cannot be discounted and warrant further investigation.
|Departments, units and centres:||Department of Pharmaceutics > Department of Pharmaceutics|
|Journal or Publication Title:||Food Technology and Biotechnology|
|Deposited By:||Library Staff|
|Deposited On:||11 May 2012 17:46|
|Last Modified:||11 May 2012 17:46|
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