Involvement of post-synaptic kainate receptors during synaptic transmission between unitary connections in rat neocortex

Ali, A.B. (2003) Involvement of post-synaptic kainate receptors during synaptic transmission between unitary connections in rat neocortex. European Journal of Neuroscience, 17 (11). pp. 2344-2350. 10.1046/j.1460-9568.2003.02677.x.

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DOI: 10.1046/j.1460-9568.2003.02677.x


The properties of functional kainate receptor-mediated EPSCs were studied in acute slices from 19-35-day-old rats. EPSCs elicited in pyramidal and fast-spiking cells in layers 2/3 and 5 of the rat motor cortex by extracellular single shock stimulus in the presence of GYK1 53655 and D-2-amino-5-phosphopentanoic resulted in a residual current. This current was not enhanced by cyclothiazide but was blocked by 6-cyano-7-nitroquinoxalin-2,3-dione and is thought to be mediated by kainate receptors. These kainate receptor-mediated currents displayed a wide range of time courses depending on which pre-synaptic fibres were activated. With paired recordings, unitary EPSCs elicited in pyramidal cells were almost totally blocked by GYK1 53655 and D-2-amino-5-phosphopentanoic. However, when L-transpyrrolidine-2,4-dicarboxylate (PDC), a glutamate uptake blocker, was introduced in the bath, the amplitude of kainate receptor-mediated currents, which is resistant to GYK1 53655 and D-2-amino-5-phosphopentanoic, was revealed. The rise and decay time constants of the kainate receptor-mediated currents were identical to control EPSCs. PDC was not required to reveal the kainate receptor-mediated currents elicited in fast-spiking cells which also displayed similar rise and decay time constants to the control EPSCs. Excitatory input onto pyramidal and fast-spiking cells in the neocortex mediated by kainate receptors contributed between 14 and 40% of the total control unitary EPSCs which displayed identical time courses to the AMPA receptor-mediated component of the EPSCs. Post-synaptic kainate receptors at connected pyramidal cell synapses may be located extra-synaptically.

Item Type:Article
Uncontrolled Keywords:GYK1; Interneurones; Motor cortex; Paired recordings; Pyramidal cells
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:3316
Journal or Publication Title:European Journal of Neuroscience
Deposited By:Library Staff
Deposited On:31 May 2012 12:39
Last Modified:31 May 2012 12:39

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