Peroxynitrite-induced toxicity in cultured astrocytes

Amin, N. and Pearce, B.R. (1997) Peroxynitrite-induced toxicity in cultured astrocytes. Brain Research, 773 (1-2). pp. 227-230. 10.1016/S0006-8993(97)00955-4.

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DOI: 10.1016/S0006-8993(97)00955-4

Abstract

The exposure of cultured astrocytes to peroxynitrite (ONOO-) for 40 min resulted in a concentration-dependent increase in the release of lactate dehydrogenase from the cells into the bathing medium over the following 24 h. Control experiments showed that the breakdown products of ONOO- contribute, to some extent, to its ability to cause cell death but that the drag vehicle (0.3 M NaOH), which increased the pH of the bathing medium to 9.4, had little effect. The cytotoxic action of ONOO- was mimicked by 3- morpholinosydnonimine (SIN-1) which liberates both nitric oxide (NO) and superoxide but not by S-nitrosoglutathione which liberates only NO, SIN-1- induced cytotoxicity was reversed in a concentration-dependent manner by superoxide dismutase and attenuated by haemoglobin suggesting that the effect of SIN-1 is due, at least in part, to the formation of ONOO-.

Item Type:Article
Uncontrolled Keywords:Astrocyte; Cytotoxicity; Nitric oxide; Peroxynitrite
Departments, units and centres:Department of Pharmacology > Department of Pharmacology
ID Code:3326
Journal or Publication Title:Brain Research
Deposited By:Library Staff
Deposited On:31 May 2012 16:41
Last Modified:31 May 2012 16:41

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