Liu, S., Griffiths, W.J. and Sjovall, J. (2003) On-column electrochemical reactions accompanying the electrospray process. Analytical Chemistry, 75 . pp. 1022-1030. 10.1021/ac026066p.
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On-Column Electrochemical Reactions Accompanying the Electrospray Process Suya Liu, William J. Griffiths,* and Jan Sjövall Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden Received for review August 22, 2002. Accepted November 15, 2002. Abstract: It is well-established that electrochemical reactions can occur within the capillary of an electrospray (ES) devise coupled to a mass spectrometer. In fact, such reactions must occur to maintain charge balance during the ES process. However, electrochemical reactions occurring distal to the capillary as a result of the high potential applied to the capillary have not been thoroughly investigated. In the present communication, we show that electrochemical processes can occur on a high performance liquid chromatography (HPLC) column coupled to an ES capillary. On-column solvent electrolysis is proposed to generate free radicals, which can subsequently initiate analyte oxidation. Oxidation of steroid sulfates possessing a reactive double bond between C-5 and C-6 is demonstrated. The possibility of similar reactions occurring within peptides possessing a site of unsaturation is also considered.
|Additional Information:||Full text available in print and electronically at the School of Pharmacy Library.|
|Departments, units and centres:||Department of Pharmaceutical and Biological Chemistry > Department of Pharmaceutical and Biological Chemistry|
|Journal or Publication Title:||Analytical Chemistry|
|Deposited By:||Library Staff|
|Deposited On:||17 Jan 2007|
|Last Modified:||19 Sep 2007 11:15|
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