Discovery of G-quadruplex stabilising ligands through direct ELISA of a one-bead-compound library

Redman, J.E., Ladame, S., Reszka, A.P., Neidle, S. and Balasubramanian, S. (2006) Discovery of G-quadruplex stabilising ligands through direct ELISA of a one-bead-compound library. Organic and Biomolecular Chemistry, 4 . pp. 4364-4369. 10.1039/b611716c.

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DOI: 10.1039/b611716c

Abstract

Discovery of G-quadruplex stabilizing ligands through direct ELISA of a one-bead-one-compound library James E. Redman, Sylvain Ladame, Anthony P. Reszka, Stephen Neidle and Shankar Balasubramanian We describe the identification of small-molecule G-quadruplex ligands using a direct ELISA screen of a one-bead-one-compound library of unnatural polyamides displayed on a branched linker with a biotin tag. This general purpose parallel screen for small molecule–oligonucleotide interactions was validated by surface plasmon resonance and ELISA of resynthesized compounds. Linear polyamides displayed similar rankings in their affinity for quadruplex as their branched counterparts. Quadruplex affinity as judged by these surface based techniques was a useful predictor of the ability of the ligands to stabilize the quadruplex to thermal unfolding in solution. Graphical abstract image for this article (ID: b611716c)

Item Type:Article
Departments, units and centres:Department of Pharmaceutical and Biological Chemistry > Cancer Research UK Biomolecular Structure Group
ID Code:779
Journal or Publication Title:Organic and Biomolecular Chemistry
Deposited By:Library Staff
Deposited On:21 Jun 2007
Last Modified:19 Sep 2007 11:16

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